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Image Search Results
Journal: Cell Death & Disease
Article Title: ZSCAN21 mediates the pathogenic transcriptional induction of α-synuclein in cellular and animal models of Parkinson’s disease
doi: 10.1038/s41419-025-07722-w
Figure Lengend Snippet: A AAV vectors expressing control shRNAs (sh-Luc and sh-eGFP) or shRNAs against Trim17 (sh-T17) and Zscan21 (sh-Z21) were unilaterally injected in the midbrain of mice. Four weeks after injection, the mRNA levels of Trim17 and Zscan21 were measured both in the contralateral and ipsilateral midbrains of each mouse by RT-qPCR using β2-microglobulin and Hmbs as reference genes. Ipsilateral/contralateral mRNA ratios are presented as box plots, with minimum, maximum and median indicated. * Significant difference (one-way ANOVA followed by Sidak’s multiple comparison test). B Four weeks after injection of AAV vectors as in A, mice were subjected to or not (saline) to subacute MPTP treatment and were sacrificed three weeks after the last MPTP injection. Brain sections were stained with anti-TH antibody by immunohistochemistry, and representative images of the ipsilateral SN in the different conditions are shown. The scale bar is 200 μm. C The total number of TH-positive neurons in the SNpc of mice was counted by stereology in the different conditions. # Significantly different from control mice (saline) injected with the same AAV (two-way ANOVA followed by Sidak’s multiple comparison test); * Significantly different from sh-eGFP in the MPTP condition (two-way ANOVA followed by Dunnett’s multiple comparison test). NB: differences are also significant when compared with sh-Luc; in saline conditions, only shT17 is different from sh-Luc or sh-eGFP. D Some brain sections from the same mice analysed in B were used for a double immunofluorescence staining of TH and GFP. Representative pictures of the ipsilateral SNpc of MPTP-treated mice are shown. For each mouse, the decrease of the total number of TH-positive neurons in the SNpc, compared to the mean of control mice (saline) injected with the same shRNA, is indicated in white. NB: in mice exhibiting a strong MPTP-induced neurodegeneration, GFP is not expressed in the SNpc, whereas in mice showing a strong neuroprotection, GFP expression is strong in the SNpc.
Article Snippet:
Techniques: Expressing, Control, Injection, Quantitative RT-PCR, Comparison, Saline, Staining, Immunohistochemistry, Double Immunofluorescence Staining, shRNA